Journal: Asian Journal of Andrology

Article Title: Expression and function of HSP110 family in mouse testis after vasectomy

doi: 10.4103/1008-682x.164197

Figure Lengend Snippet: Figure 2: Vasectomy induces changes in mRNA and protein levels of HSP110 family members. (a) The level of HSP110 mRNA in the mouse testis was determined by RT‑PCR. (b) Quantification of ORP150 and HSPA4l mRNA in vasectomized and control mice relative to β‑actin. Control, one representative of sham‑operated control (note that sham‑operated controls at each time point postvasectomy had the same expression level for each HSP110 gene). *Statistically significant difference for vasectomy versus sham operation of the same day (P < 0.05). Results of one representative experiment of two others. (c) Expression of HSP110 in mouse testes at different time points postvasectomy detected by Western blot. GAPDH was assessed as an internal loading control. Control, one representative of sham‑operated control (note that sham‑operated controls at each time point postvasectomy had the same expression level for each HSP110 protein). Results of one representative experiment of two others.

Article Snippet: The primary antibodies used were: GAPDH (sc-25778: Santa Cruz Biotechnology, Santa Cruz, CA, USA); HSPA4 (sc-365366: Santa Cruz Biotechnology, Santa Cruz, CA, USA); HSPA4l, ORP150, and HSPH1 (ARP53693, ARP46716, ARP53693: Aviva Systems Biology, Beijing, China); phospho-JNK, phospho-eIF2α, JNK, eIF2α, GRP78, and GADD153 (Cell Signaling Technology, Danvers, MA, USA).

Techniques: Control, Expressing, Western Blot

Journal: Frontiers in Endocrinology

Article Title: Maternal Protein Restriction Induces Alterations in Hepatic Unfolded Protein Response-Related Molecules in Adult Rat Offspring

doi: 10.3389/fendo.2018.00676

Figure Lengend Snippet: Determination of differentially expressed hepatic UPR-related genes in IUGR foetal livers. (A) qPCR analysis showing the mRNA expression of hepatic UPR-related genes in foetal rats. Data are expressed as mean ± SEM. * p < 0.05 vs. age-matched controls ( n = 8). (B) Representative agarose gel electrophoresis images of PCR products confirmed the amplicon size and specificity. (C–H) Histological expression of UPR factors in the fetal hepatics. Representative photomicrographs of IHC staining for heat shock protein a4l (Hspa4l) (C,D) , mitogen-activated protein kinase 10 (Mapk10) (E,F) and Creb3l3 (G,H) in the liver sections from control and IUGR rats (Magnification: 400×; scale bar = 50 μm). At E20, hepatic Hspa4l and Mapk10 protein levels was decreased in IUGR foetuses compared to age-matched controls, while Creb3l3 increased in fetal livers.

Article Snippet: The primary antibodies used for IHC staining included rabbit anti-heat shock protein A4L (HSPA4L) (1:100, orb184242, Biorbyt, Cambridge, United Kingdom), anti-CREB3L3 (1:300, YN1809, Immunoway, Plano, TX, United States), and anti-mitogen activated protein kinase 10 (MAPK10) (1:100, E1A5321, Enogene, Nanjing, China).

Techniques: Expressing, Agarose Gel Electrophoresis, Amplification, Immunohistochemistry, Control